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Keywords: ulcerative colitis, GHK-Cu, SIRT1/STAT3 pathway, co-culture system, network pharmacology, molecular docking 1 Introduction Ulcerative colitis (UC) is a chronic, relapsing form of inflammatory bowel disease (IBD) characterized by immune system dysfunction and pathological overactivation of mucosal immune responses, leading to persistent inflammation and subsequent colonic mucosal injury (Raine et al., 2022)

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Injectable protocols typically use smaller amounts due to higher absorption efficiency