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After 48 h, qRT-PCR was used to evaluate the KD efficiency of each shRNA sequence

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In conclusion, we revealed novel structure–function relationships of glutaredoxins, gained insights regarding the enzymatic conversion of glutathione- and non-glutathione disulfide substrates, and identified two distinct substrate interaction sites that include a scaffold residue and the conserved dual activator Lys105 in ScGrx7 and Lys26 in PfGrx

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