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Analogous precipitates from chymotrypsin-treated RBC yielded primarily a labeled 65 kDa fragment, implying that the intact antigen was band 3 (the two chymotryptic fragments of band 3 were ascribed the MW of 65 and 38 kDa to the carbohydrate-containing fragment, later with sequence data available these fragments were named 55 and 35 kDa fragment)

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