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therefore, MeCbl remains an investigational, mechanism-focused approach in ASD rather than an established treatment.8 Overall, intramuscular and subcutaneous MeCbl is generally well tolerated

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The samples were withdrawn at the time intervals 0, 1, 10, 30, 60, 120, 240, 480, and 1440 min and mixed with equal volume of ice-cold HCl (0.2 molL −1 ) until analysis by HPLC