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In the morning, each participant filled out a Hangover Symptom Scale questionnaire, evaluating each hangover symptom (feeling thirsty or dehydrated, feeling more tired than usual, headache, nauseated, vomited, feeling weak, difficulty concentrating, more sensitive to light and sound than usual, sweating more than usual, had trouble sleeping, feeling anxious, feeling depressed, experienced trembling or shaking) on a 5 point Likert scale with 1 representing “Strongly Disagree” and 5 representing “Strongly Agree”

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The cells were first blocked with 5 μg anti-CD16/CD32 mAb for 5 min, and then stained with 0.5 μg of APC-labeled anti-mouse CD11b mAb, 2 μg of PE-Cy5.5-labeled anti-mouse CD45.2 mAb, 1 μg of Brilliant violet 421-labeled anti-mouse Ly6G, 1 μg Brilliant violet 785-labeled anti-mouse Ly6C, and 2.5 μg Alexa Fluor 488-labeled anti-mouse F4/80 on ice for 10 min