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Following 3× 10min washes with 1ml 0,2% TBS-Gelatin and 1 wash with regular TBS the slides were mounted using soft set mounting medium with DAPI (Vectashield) and sealed with nail polish

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Two things follow: the chemical identity of what is in the well is no longer what was weighed out, and the oxidative stress generated can itself change transcription and proliferation in ways that look like compound activity