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Addition of dithiothreitol (a thiol reducing agent) restored complex I activity to control levels in GCL-diminished cells, suggesting that it is the oxidation of sulfhydryl groups that results in the inhibition of complex I

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Immunofluorescence assays For all immunofluorescence assays, cells (typically 15,000) were seeded per well of 8-well plates (SPL 30408)