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As discussed in the FY 2003 IPPS/LTCH PPS final rule (67 FR 49993 through 49994), the procedure code for the insertion of totally implantable vascular access devices was added to the GROUPER logic of DRG 315 (Other Kidney and Urinary Tract O.R

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The cells were diluted to a certain density and then moved to polylysine (Sigma, P1399)-coated plates for culturing at 37 °C in a 5% CO 2 incubator