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The elution profile of the sample homogenized in BSAN buffer (blue tracing) is characterized by the presence of both the two symmetrical peaks corresponding to the GS-NEM conjugate (4.29 min and 4.95 min r.t.) and the peak corresponding to the excess of NEM (5.8 min r.t.)

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This would result in the formation of neurofibrillary tangles (NFTs) and the subsequent disruption of normal neuronal and synaptic functions [95]