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Hydrogen peroxide and redox modulation sensitize primary mouse hepatocytes to TNF-induced apoptosis

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For measuring O 2 −• levels, the reaction system contained 50 mM phosphate buffer (pH 7.8) supplemented with 17 mM sulphanilic acid, 10 mM hydroxylamine hydrochloride, and 7 mM 1-naphthylamine, with absorbance determined at 530 nm (Liu et al., 2007)