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280 Furthermore, activation of the inflammation-related NLRP3 inflammasome signaling pathway in different types of neural cells was closely associated with high cholesterol load, which triggered neuroprotective properties in activated microglia but promoted oxidative stress in neurons, further enhancing the expression of NLRP3 inflammasomes, inducing neuronal pyroptosis, and impairing the phagocytic capacity of microglia
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Briefly, mouse liver was pre-perfused with Leffert’s buffer (HEPES 10 mM, KCl 3 mM, NaCl 130 mM, NaH 2 PO 4 1 mM, glucose 10 mM, pH 7.4) containing EGTA, followed by perfusion with Liberase that consists of collagenase I and II